A new mode to light up adjacent DNA-scaffolded silver probe pair and its application for specific DNA detection

Reagents. Oligonucleotides were synthesized and purified by Sangon Inc. (Shanghai, China). The sequences of these oligonucleotides are listed in Table S1. E.coli exonuclease III (Exo III) was purchased from Fermentas Inc. (Vilnius, Lithuania). Silver nitrates (AgNO3) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Sodium borohydride (NaBH4) was obtained from Tianlian Fine Chemical Co., Ltd. (Shanghai, China). Other chemicals were analytical grade and directly used without additional purification, purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). All solutions were prepared with ultrapure water (18.2 MΩcm) from a Millipore Milli-Q system (Bedford, MA, USA). The reaction buffers used in DNA/AgNCs synthesis were as follows: 2 MOPS (100 mM NaNO3, 40 mM MOPS, pH 7.0), 2 HEPES (20 mM HEPES, 200 mM NaNO3, pH 7.4), 2 Na3PO4 (40 mM Na3PO4, pH 6.6), 10 Tris (100 mM Tris, pH 7.9).